Resources

Graphical Interpretation and Analysis of Proteins and their Ontologies (Gia Pronto) data analysis and visualization software
JoVE video of protocol for histone isolation and preparation for Bottom Up MS

Protocol for nuclei isolation and acid extraction of histones
Protocol for propionylation of histones
Our EpiProfile software (EpiProfile 2.0: A Computational Platform for Processing Epi-Proteomics Mass Spectrometry Data. Yuan ZF, Sidoli S, Marchione DM, Simithy J, Janssen KA, Szurgot MR, Garcia BA. J Proteome Res. 2018 Jul 6;17(7):2533-2541) to quantify histone PTMs is freely available upon request (please send an email to [email protected])

To discuss obtaining our heavy isotope labeled synthetic histone peptide library (Lin et al. Mol Cell Proteomics. 2014, 13(9), 2450-2466), please contact Ben Garcia ([email protected])
Quick guide protocol for Bottom Up Proteomic Analysis
Histone Proteomics Formal Protocols (Karch et al. Methods in Enzymology, 574, 3-29, 2016)